Staff members at the region's ambulance trust could strike over proposed changes which would see team-based working introduced.
More than 1,000 GMB members could vote on strike action at the end of September if talks with the East of England Ambulance Service Trust (EEAST) over the amendments do not progress.
The trust is proposing to bring in team-based working, which the union says would involve redesigning rotas and removing long-term working relationships.
It has prompted GMB officials to call on the trust to decide "between their vanity project and staff welfare."
Ashley Foster, GMB regional organiser, said: “Our members are worried about the impact these changes could have on patient safety and their working relationships.
“We have explained our members’ concerns to the Trust, but we cannot force them to listen. Ultimately the Trust must decide between their vanity project and staff welfare."
According to the union, an indicative ballot held following the announcement saw 80% of participating members vote in favour of industrial action on an 80% turnout.
The trust say the changes would mean staff working within a small team, with a dedicated line manage and team leader on shift with their team.
"We understand the concerns raised by GMB and are committed to working closely with our staff and unions to get this right," said Mr Moloney.
"Team-based working is being designed to better support our people, ensuring everyone has access to a compassionate manager and is part of a supportive, collaborative team.
"Above all, patient safety remains our top priority. Any changes will be carefully planned to ensure we continue delivering the highest standards of care."
Talks are ongoing to avoid industrial action, but the union has warned that if no progress is made, ballots could be sent to members before the end of September.
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